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. 2017 Oct 1;144(19):3440–3453. doi: 10.1242/dev.154971

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© 2017. Published by The Company of Biologists Ltd

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Fig. 1. — The shielded irradiation assay. (A-C) Point source X-ray irradiator (A) passing through a lead shield (C) with aligned Schmidtea mediterranea worms (B) that have been anesthetized in 0.2% chloretone. (D) Wild-type (WT) unirradiated planarians showing distribution of NBs (green) and their early progeny (magenta). (E) Striped planarians at 4 days post irradiation (dpi) showing bands of stem cells (green) and early progeny (magenta) restricted to the irradiation-protected region. (F) Loss of NBs (green) and early progeny (magenta) in the non-shielded region after 1, 2, 3 or 4 dpi (n=10), and maintenance within the shielded region. See also Fig. S1.