Fig. 3.

Epidermal lineage cell migration. (A) Current model of planarian epidermal lineage differentiation. Question marks indicate that currently there is no direct evidence demonstrating direct transitions from one cell type to another. (B-K) WFISH showing migration of the epidermal lineage at 7 dpa. (B,C) agat-1⁺ cells (magenta) and smedwi-1⁺ cells (green). (D,E) prog-1⁺ cells (magenta) and smedwi-1⁺ cells. (F,G) prog-1⁺ cells (magenta), zeta class cells (green) and prog-1⁺/zeta class double-positive cells (white). (H,I) smedwi-1⁻ zeta class cells (magenta) and smedwi-1⁺ zeta stem cells (white) and smedwi-1⁺ cells (green). (J,K) smedwi-1⁺ sigma stem cells (white) and smedwi-1⁺ cells (green) migrate. Arrowheads indicate examples of double-positive cells. The shielded region is beneath the dotted line. Scale bars: 300 μm in B,D,F,H,J; 100 μm for C,E,G,I,K. (L) Distance traveled by the ten most distal cells in each population (smedwi-1⁺ sigma class stem cells, smedwi-1⁺ zeta class stem cells, smedwi-1⁻ zeta class cells, prog-1⁺/zeta class double-positive cells, prog-1⁺ cells and agat-1⁺ cells) in decapitated worms at 7 dpa. n=15 per condition. Student's t-test, *P<0.05. (M) Summary of migration and differentiation data after wounding.