Fig 3. Characteristics of EWS/FLI-bound microsatellites.

(A) Permutation test shows that the number of EWS/FLI binding sites that overlap with repeat regions (n = 8,256) with minimum of 3 consecutive motifs is significantly higher than random chance (p < 0.001). Red line denotes the significance limit (α = 0.05). Gray bars represent the number of overlaps in the random regions with EWS/FLI binding sites in 1,000 permutations. The black line represents the mean of overlaps in random regions (EV_perm) and the green bar is the actual number of overlaps observed in repeat regions (Obs). (B) Boxplot of EWS/FLI fold-enrichment (relative to genomic background) and number of consecutive motifs in EWS/FLI-bound microsatellites showing statistically significant increasing trend (p < 2.2 × 10⁻¹⁶). The blue line is the estimated LOESS regression line of the mean with the estimated 95% confidence bands (shaded region). (C) Boxplot of EWS/FLI fold-enrichment and total number of motifs in EWS/FLI-bound microsatellites showing a positive correlation (p = 1.9 × 10⁻¹⁰) and a non-linear trend (p < 0.05). The blue line is the estimated LOESS regression line of the mean with the estimated 95% confidence bands (shaded region). (D) Boxplot of EWS/FLI fold-enrichment and Density $(=\frac{totalmotif\times 4}{lengthofmicrosatellite}\times 100\%)$ showing statistically significant positive correlation (p < 2.2 × 10⁻¹⁶). The blue line is the estimated LOESS regression line of the mean with the estimated 95% confidence bands (shaded region).