Skip to main content
. 2017 Nov 1;12(11):e0187229. doi: 10.1371/journal.pone.0187229

Fig 4. Dry matter degradation (DMD) of forages and fibrolytic ruminal population.

Fig 4

(A) Dry matter degradation of alfalfa hay (AH) and corn silage (CS) by the rumen microbiota after 24 hours of incubation was quantified in the Daisy II incubation system containing RF in the presence or absence of L. reuteri LB1-7 and 80 mM glycerol. Non-incubated bags containing forage were used as control (see the experimental procedures section). The data are expressed as the percentage of dry matter degraded. Bars represent the SEM of three independent experiments. (B) Quantification of the rrs gene copies of the total rumen bacterial population, F. succinogenes and R. flavefaciens in DAISY II vessels. Total DNA template extracted from the Daisy II vessel containing only RF and buffer was used as control (Ctrl). The bacterial populations were quantified before and after 24 hours of incubation. pH was monitored at the start and end of incubation. Bars represent the SEM of three independent experiments. Asterisks indicate statistical significance (*: P<0.05; ***: P<0.001).