Fig 5. Growth or survival of EHEC in rectum contents after incubation in RF.
The strain FCH6 RifR was first incubated in filter-sterilized RF (FS-RF) samples alone or inoculated with ≈ 107 CFU/mL of L. reuteri LB1-7 supplemented with 80 mM glycerol under anaerobiosis for 24 hours. The bacterial pellet was then inoculated into Rec samples and incubated under anaerobiosis. RF = 0 represents inoculation of EHEC in FS-RF samples; Rec t = 0 corresponds to RF t = 24h i.e. number of EHEC surviving the incubation in FS-RF during 24 hours; Rec t = 6h and Rec t = 24h correspond to EHEC survival in Rec samples after 6 and 24 hours of incubation respectively. Bars represent the SEM of three independent experiments. Effect of L. reuteri + Glyc80 is significant *, P<0.05; ***, P<0.001.