Table 1. Concentration of glycerol and microbial metabolites.
| Acetate | Propionate | Butyrate | Glycerol | HPA | 1,3-PD | Lactate | |||
|---|---|---|---|---|---|---|---|---|---|
| (mM)a | (mM)a | (mM)a | (mM)b | (mM) | (mM) | (mM) | |||
| RF t = 0 | 61.2 ± 5.5 | 14.1 ± 1.6 | 6.3 ± 0.7 | - | - | - | - | ||
| RF t = 24h | 105.3 ± 2.9 | 33.7 ± 0.8 | 19.0 ± 1.1 | - | - | - | - | ||
| RF + LB1-7 t = 24h | 95.0 ± 2.4NS | 29.9 ±0.9* | 17.5 ± 1.8NS | - | - | - | 1.8 ± 0.09 | ||
| RF-Glyc80 t = 0 | 61.2 ± 5.5 | 14.1 ± 1.6 | 6.3 ± 0.7 | 82.0 ± 3.5 | - | - | - | ||
| RF-Glyc80 t = 24h | 95.8 ± 4.1NS | 40.8 ± 1.6* | 26.1 ± 1.7* | 61.9 ± 5.8 | - | 2.6 ± 0.9 | 0.9 ± 0.35 | ||
| RF-Glyc80 + LB1-7 t = 24h | 79.6 ± 4.8* | 17.8 ± 1.1*** | 7.8 ± 1.2** | 34.2 ± 2.1* | 7.3 ± 1.7 | 34.3 ± 2.8 | 28.7 ± 5.7 | ||
| RF-Glyc80 + 100–23 t = 24h | ND | ND | ND | 46.1 ± 1.9NS | - | 3.5 ± 0.5 | ND | ||
Concentrations of major ruminal fermentation ends products, glycerol, HPA and 1,3-PD were quantified in RF and RF-Glyc80. The samples were inoculated or not with L. reuteri LB1-7. The detection limit of glycerol, 1,3-PD, lactate and HPA were 102 mM, 2 x 102 mM,102 and 5 x 10−2 mM, respectively. Means of three independent replicates are shown with their SEM. ND: not determined; -: undetected.
a: comparisons were made with RF t = 24h
b: comparisons were made with RF-Glyc80 t = 24h
NS: P>0.05 (not significant)
*: P<0.05
**: P<0.01
***: P<0.001.
Values with no superscript were not statistically analyzed.