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. 2017 Nov 1;12(11):e0187523. doi: 10.1371/journal.pone.0187523

Fig 4. Comparing buffers B1 and B2 with liver mitochondria.

Fig 4

Liver mitochondria were loaded in the oxygraph chambers at a concentration of 0.5 mg/ml. The substrate combination PM was used to test the effect of buffers B1 and B2 on respiration. (A) Representative dynamics for liver mitochondrial respiration in buffers B1 and B2. When leak state respiration stabilized, 500 μM ADP was added to simulate oxidative phosphorylation. (B) Comparison of leak state respiration rates, ADP-stimulated respiration rates, and RCRs. Leak state respiration rates were averaged over a 30-second window just prior to ADP addition. ADP-stimulated and FCCP-uncoupled respiration rates were averaged over a 30-second window covering the peak of each respiration dynamic. Data presented as mean +/- standard deviation; ** indicates significance level of p<0.01.