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Splenocytes from naïve 13 week old ApoE(-/-) mice were stimulated with 2 or 20μg/ml truncated(t) CRAMP peptide for 24 hours (A-D) or 48 hours (E-H). Analysis of cell stains was based on the gating scheme depicted in S3 Fig. Bars over graphed columns indicate statistical significance (P<0.05; N = 4 each).
