Fig 8. T cell activation in Cramp-immunized mice.

Splenocytes from Cramp 100μg dosed mice (A-D) or Cramp 20μg dosed mice (E-H) were collected one week after the last booster injection and challenged with 20μg/ml tCRAMP for 24 hours and stained for T cell activation markers. Gating is as depicted in S3 Fig. Bar over graph columns indicate statistical significance; P<0.05. Mean of 3 separate experiments with at least 2 mice pooled per group in 3–6 replicates per experiment.