Figure 6.

Effect of ALA on proinflammatory cytokines and chemokines. (A) M-CSF (30 ng/mL) and RANKL (100 ng/mL) treated BMMs were cocultured with indicated concentrations of ALA for 3 days. Cells were then harvested, RNA isolated, cDNA synthesized, and qPCR performed. Quantification of gene was presented relative to normalized control (1.0 fold). ^ P<0.012 vs. control, * P<0.032 vs. RANKL. (B) Systemic inflammation assessment from LPS-challenged mice. A multiplex Mouse Cytokine Immunoassay was performed and quantity of proteins was presented in pg/ml serum. ^ P<0.012 vs. control, * P<0.032 vs. RANKL.