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. 2017 Oct 24;23:5070–5082. doi: 10.12659/MSM.906959

Figure 7.

Figure 7

(A) Images of nuclei shrinking and evident nuclear condensation of osteosarcoma (OS) cells (shown by arrowhead) after mechanical stretch forces of 0, 2, 12, 24, and 48 hours. Notes: (a) 0-hour group 200×. (b) 0-hour plus shPiezo1RNA group 200x. (c) 2-hour group 200×. (d) 2-hour plus shPiezo1RNA group 200×. (e) 12-hour group 200×. (f) 12-hour plus shPiezo1RNA group 200×. (g) 24-hour group 200×. (h) 24-hour plus shPiezo1RNA group 200×. (i) 48-hour group 200×. (j) 48-hour plus shPiezo1RNA group 200×. (B) Results of flow cytometry detection of 0S cell apoptosis in 0, 2, 12, 24, and 48 hours and shPiezo1RNA groups. Q1: (AnnexinV-FITC)−/PI+, necrotic cells. Q2: (AnnexinV-FITC)+/PI+, late stage apoptotic cells. Q3: (AnnexinV-FITC)+/PI−, early stage apoptotic cells. Q4: (AnnexinV-FITC)−/PI−, live cells. Notes: (a) 0-hour group. (b) 0-hour group plus shPiezo1RNA. (c) 2-hour group. (d) 2-hour group plus shPiezo1RNA. (e) 12-hour group. (f) 12-hour group plus shPiezo1RNA. (g) 24-hour group. (h) 24-hour group plus shPiezo1RNA. (i) 48-hour group. (j) 48-hour group plus shPiezo1RNA. (C) Early stage apoptosis of human OS cells in 0, 2, 12, 24, 48 hors and shPiezo1RNA groups. After a 2 hour mechanical stretch, the rate of early stage apoptosis significantly increased. After a 12 hour mechanical stretch, the late stage apoptotic rate increased and peaked in the 24-hour group. The late stage apoptotic rate was lower in the 48-hour group compared to the 24-hour group. The results are presented as the means ±SD, n=3; ns means p>0.05 versus the control group; * p<0.05 versus the control group; ** p<0.01 versus the control group; one-way ANOVA.