Supplementary Figure S3.

Thermal stability of mutants by CD. Complete temperature unfolding profile at 222 nm demonstrated distinct unfolding nature of wild-type BAP1N shown in red and mutants shown in blue. Melting temperatures were obtained from sigmoidal fits over the range of 25 °C to 75 °C controlled by Peltier control system using 0.2 cm path length cuvette. The protein concentration was 7 μM in 50 mM Tris, pH 7.4 and 150 mM NaCl. Solid line represents the sigmoidal curve fitting.

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