Figure 5. Sensograms for the binding of BAP1N mutants to ubiquitin.

BAP1N C91S and other double mutants of concentration ranging from 0 to 50 μM in 50 mM Tris, pH 7.4 and 150 mM NaCl were perfused over ubiquitin immobilized covalently on a hydrophilic carboxymethyl-dextran matrix (Sensor Chip CM5). Representative sensograms show binding profiles of all the proteins.