Supplementary Figure S1.

Multiple sequence alignment of BAP1 with human UCHL5, UCHL1, UCHL3 and TsUCH37. Structure based sequence alignment of UCH domain of BAP1 with other known human UCHs and Trichinella spiralis ubiquitin hydrolase TsUCH37. Catalytic residues of BAP1 (blue colored asterisks), conserved among the UCHs and TsUCH37, are highlighted in blue. Residues highlighted in yellow are important for ubiquitin binding, curated from the ubiquitin bound crystal structure data of human UCHL1, UCHL3, UCHL5 and TsUCHL5. The proposed BAP1N residues interacting with ubiquitin are shown with red colored asterisks and similar residues in other UCHs highlighted in red. Active site crossover loop residues are highlighted in green.

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