Table 2. Binding and thermodynamic parameters for UCHL1 C90S, UCHL3 C95S, UCHL1 C88S, and BAP1 C91S in 50 mM Tris, pH 7.4 and 50 mM NaCl.
| Parameters | Proteins | |||
|---|---|---|---|---|
| L1 C90S | L3 C95S | L5 C88S | BAP1 C91S | |
| ΔH (kJ/mol) | –27.3 ± 0.5 | –49.6 ± 1.6 | –18.6 ± 1.3 | –10.9 ± 1.1 |
| n | 0.9 ± 0.1 | 0.8 ± 0.02 | 1.2 ± 0.1 | 0.6 ± 0.4 |
| Kd (μM) | 0.2 ± 0.1 | 0.01 ± 0.001 | 10.1 ± 3.6 | 3.2 ± 1.0 |
| ΔS (J/mol·K) | 37.3 ± 4.4 | –14.9 ± 5.6 | 33.2 ± 0.5 | 68.6 ± 2.7 |
| ΔG (kJ M−1) | –38.3 ± 0.7 | –45.3 ± 0.3 | –28.3 ± 1.2 | –31 ± 0.6 |
Kd, dissociation constant; n, binding stoichiometry; ΔH, change in enthalpy; ΔS, change in entropy.