Figure 2. Analysis of reverse transcription products.
(A) Partial DNase I digestion of full-length products obtained using template 1, in comparison to authentic materials, with either an RNA primer (left 7 lanes) or DNA primer (right 7 lanes). For the RNA-primed reaction, only the extended portion is cleaved; for the DNA-primed reaction, both the primer and extended portion are cleaved. (B,C) LC/MS analysis of purified full-length products obtained using template 1 and either an RNA or a DNA primer, respectively.
Figure 2—source data 1. LC/MS analysis of full-length products.
elife-31153-fig2-data1.xlsx (94.4KB, xlsx)
DOI: 10.7554/eLife.31153.008
Figure 2—figure supplement 1. High-resolution MS/MS analysis of reverse transcription product.
Extension of an all-RNA primer on template four yielded a product with a calculated mass of 8874.432 and observed mass of 8874.441. The parent ion was fragmented at internucleotide linkages within the DNA portion of the molecule to generate secondary ions that were analyzed by tandem MS. Successive fragments a–h correspond to 3´-terminal subsequences within the DNA portion of the molecule.
Figure 2—figure supplement 1—source data 1. High-resolution MS/MS analysis of reverse transcription product (related to Figure 2—figure supplement 1).
elife-31153-fig2-figsupp1-data1.xlsx (51.4KB, xlsx)
DOI: 10.7554/eLife.31153.009