Key resource table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Sacccharomyces
cerevisiae) |
HMS2 | YJR147W; SGD ID S000003908; RRID:SCR_003093; SGD, RRID:SCR_004694 |
||
| Gene (S. cerevisiae) | GAL1 | YBR020W; SGD ID S000000224; RRID:SCR_003093; SGD, RRID:SCR_004694 |
||
| Gene (S. cerevisiae) | BAT2 | YJR148W; SGD ID S000003909 RRID:SCR_003093; SGD, RRID:SCR_004694 |
||
| Gene (S. cerevisiae) | snR52 | SGD ID S000006443; RRID:SCR_003093; SGD, RRID:SCR_004694 |
||
| Strain, strain background (S. cerevisiae) |
see Supplementary file 1C | see Supplementary file 1C |
Saccharomyces cerevisiae, NCBITaxon:1247190; SGD, RRID:SCR_004694 |
see Supplementary file 1C |
| Genetic reagent (S. cerevisiae) |
see Supplementary file 1C | see Supplementary file 1C | see Supplementary file 1C | see Supplementary file 1C |
| Antibody | Tubulin (rat monoclonal YL1/2) |
Abcam | Abcam ab6160 RRID:AB_305328 |
(1:3333) |
| Antibody | Cas9 (rabbit polyclonal) | Diagenode | C15310258 RRID:AB_2715516 |
(1:5000 Western blot); 5 µl/ChIP |
| Recombinant DNA reagent |
see Supplementary file 1A | Eurofins Genomics | see Supplementary file 1A | see Supplementary file 1A |
| Recombinant DNA reagent |
Plasmid with pTDH-dCas9 |
(Qi et al., 2013) Addgene | Addgene #46920 RRID:SCR:002037 |
PMID:23849981 |
| Sequence-based reagent |
single-stranded DNA templates for variable and constant sgRNA regions |
This study | see Supplementary file 1A | synthesised by phosphoramidite oligonucleotide synthesis with 3’-alkyne or 5’-azido modifications (El-Sagheer et al., 2011) |
| Sequence-based reagent |
see Supplementary files 1A,B,D,E,F | Eurofins Genomics | see Supplementary files 1A,B,D,E,F | see Supplementary files 1A,B,D,E,F |
| Commercial assay or kit |
QIAquick PCR Purification Kit |
Qiagen; RRID:SCR_008539 | Cat# 28106 | |
| Chemical compound, drug |
SYBR green qPCR master mix |
Bioline | Cat# QT605-20 | |
| Software, algorithm | Off-spotter sgRNA algorithm |
https://cm.jefferson.edu/Off-Spotter// | ||
| Software, algorithm | Rotor-Gene Q Series Software |
https://www.qiagen.com/us/resources/ | ||
| Software, algorithm | Fiji/Image J | https://imagej.net/Fiji | ImageJ, RRID:SCR_003070 |