Video 5. Dynamics of Vps4^E233Q-eGFP recruitment to endosomes of yeast cells visualized using LLSM.

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DOI: 10.7554/eLife.31652.010

Related to Figure 3. The 51 s 3D time series acquired using LLSM of yeast cells expressing Vps4 ^E233Q-eGFP. The movie shows a deconvolved and rendered 3D view and orthogonal deconvolved side views. The fluorescent objects were traced using the automated 3D detection and tracking software and then color coded as rainbow from blue to red following strength of the signal.