Video 6. Effect of the Vps4^E233Q mutant on the dynamics of Snf7-eGFP recruitment to endosomes of yeast cells visualized using LLSM.

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DOI: 10.7554/eLife.31652.011

Related to Figure 3. Cells expressing a mixture of Snf7 and Snf7-eGFP together with Vps4^E233Q-mCherry were imaged for 51 s. Although all the fluorescence signals of Snf7-eGFP and Vps4^E233Q-mCherry colocalized (Figure 3), for simplicity, the movie only shows the fluorescence for Snf7-eGFP. The movie shows a deconvolved and rendered 3D view and orthogonal deconvolved side views. The fluorescent objects were traced using the automated 3D detection and tracking software and then color coded as rainbow from blue to red following strength of the signal.