Table 1.
Primers and probes included in the in-house M55 multiplex PCR master mix.
| Primers and probes | Sequence (5′–3′)a | Refs. |
|---|---|---|
| fliD-F | TGGTGACAGAGTGTATGATAATGGAA | Hojgaard et al. (2014) |
| fliD-R | ACTCCTCCGGAAGCCACAA | Hojgaard et al. (2014) |
| fliD-probe | FAM-TGCTAAAATGCTAGGAGATTGTCTGTCGCC-BHQ1 | Hojgaard et al. (2014) |
| 23S-F | TCGGTGAAATTGAAGTATC | Johnson et al. (2017) |
| 23S-R | CARGCTATAGTAAAGGTTCA | Johnson et al. (2017) |
| 23S-probe | HEX-CGTCTAACCACAAGTAATCGGCATC-BHQ1 | Johnson et al. (2017) |
| purB-F | TCCTCAATGATGAAAGCTTTA | Graham et al. (2016) |
| purB-R | GGATCAACTGTCTCTTTAATAAAG | Graham et al. (2016) |
| purB-probe | CalRd610-TCGACTTGCAATGATGCAAAACCT-BHQ2 | Graham et al. (2016) |
| actin-F | GCCCTGGACTCCGAGCAG | Hojgaard et al. (2014) |
| actin-R | CCGTCGGGAAGCTCGTAGG | Hojgaard et al. (2014) |
| actin-probe | Q670-CCACCGCCGCCTCCTCTTCTTCC −BHQ3 | Hojgaard et al. (2014) |
a
BHQ1, BHQ2, BHQ3: Black Hole Quencher 1, 2 and 3 respectively; CalRd610: CalFluor Red 610; FAM: 6-carboxyfluorescein; HEX: hexachlorofluorescein phosphramidite; Q670: Quasar 670.