Fig. 3.

LKO confers on HFD the ability to decrease liver 2-MGs in male mice. Male and female WT and FABP1 LKO mice on a C57BL/6N background were pair-fed a control diet (black bars, CO) or HFD (open bars, HF), as described in the Materials and Methods. All conditions were as in Fig. 2 except that 2-MGs were quantified by LC-MS analysis using deuterated internal standards (Cayman Chemical), as described in the Materials and Methods. Shown are 2-arachidonoylglycerol (2-AG) (A), 2-OG (B), and 2-palmitoylglycerol (2-PG) (C). Mean ± SEM (n = 8). By ANOVA, *P ≤ 0.05 HFD versus control diet; ^#P ≤ 0.05 LKO versus WT on same diet; ^{^}P ≤ 0.05 female versus male of same genotype and diet.