Fig. 4.

Effect of LKO on ability of HFD to impact liver protein levels of membrane FA transport/translocase proteins involved in uptake of FA. Male and female WT and FABP1 LKO mice on a C57BL/6N background were pair-fed a control diet (black bars, CO) or HFD (open bars, HF), as described in the Materials and Methods. All conditions were as described in Fig. 2 except that SDS-PAGE followed by Western blotting on aliquots of liver homogenates were done, as described in the Materials and Methods, to determine levels of: FATP5 (A), FATP2 (B), and FATP4 (C). Relative protein levels were normalized to gel-loading control protein COX4 (FATP5 and FATP2) and β-actin (FATP4) and values compared with male WT set to 1. Mean ± SEM (n = 6–7). By ANOVA, *P ≤ 0.05 HFD versus control diet; ^#P ≤ 0.05 LKO versus WT on same diet; ^{^}P ≤ 0.05 female versus male of same genotype and diet.