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. 2017 Aug 19;58(11):2147–2161. doi: 10.1194/jlr.M079764

Fig. 3.

Fig. 3.

Accumulation of oleic acid in Plin2+/+ and Plin2−/− myotubes. Myotubes were incubated with [1-14C]OA (100 or 400 µM) and accumulation over 24 h was determined with scintillation proximity assay. Accumulation was determined in presence of DMSO (0.1%) (A, B) or in presence of the adipose triglyceride lipase inhibitor (Atglistatin, 10 µM) (C, D). The results are presented as means ± SEM (n = 3, *P < 0.05 vs. Plin2+/+ across all points in time). E: The effect of Atglistatin on accumulation of [1-14C]OA assessed as an average of all time points from A–D. F: Cell-associated [1-14C]OA after 24 h incubation with 100 μM OA in presence of the lipase inhibitor (CAY10499, 10 μM) or CAY10499 combined with Atglistatin (10 μM). For E and F, the results are presented as means ± SEM normalized to DMSO treated Plin2+/+ myotubes (n = 3, #P < 0.05 vs. DMSO).