Table 2.

Timing of collection, processing and/or main extraction for AMANHI biological samples

Sample type	Timing of collection	Processing & planned use of the sample
Maternal blood	Enrolment, 24–28 weeks or 32–36 weeks, postnatal day 42–60	For DNA extraction, HbA1C analysis, and serum/plasma extraction, aliquoting and storage
Maternal urine	Enrolment, 24–28 weeks or 32–36 weeks, postnatal day 42–60	Uncentrifuged and centrifuged sample, biochemical and pathological analysis
Cord blood sample	At birth	DNA extraction, HbA1C analysis, and serum/plasma extraction, aliquoting and storage
Cord tissue samples	At birth	RNALater, alcohol, flash frozen and formalin sample
Placenta tissue samples	At birth	RNALater, alcohol, flash frozen and formalin sample
Placenta membrane samples	At birth	RNALater, alcohol, flash frozen and formalin sample
Maternal faeces	At birth	Maternal faecal microbiome
Paternal saliva	Antenatal or postnatal	Paternal DNA
Fetal faeces	Postnatal day 42–60	Newborn faecal microbiome