Cumulus cells were treated for 48 hours with vehicle (C), GDF9 (2.5, 10ng/ml), BMP15 (2.5, 10ng/ml), or the combination of GDF9 and BMP15 (0.1, 0.6, 2.5, 5, or 10ng/ml of each). Amh mRNA levels were determined by qPCR and expressed as relative to Rpl19. Columns represent the mean ± SEM, Columns with different letters differ significantly a–b P<0.05, a–c P<.001 vs controls, one-way ANOVA, Bonferroni test, n=15.