Cumulus cells were treated for 48 hours with GDF9 and BMP15 (G+B; 5 ng/ml of each) in the presence or absence of FSH (50 ng/ml). Amh mRNA levels were determined by qPCR and expressed relative to Rpl19. Columns with different letters differ significantly a–b P<0.001, a–c P<0.05, b–c P<0.01, one-way ANOVA, Bonferroni test, n=11.