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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Reproduction. 2017 Sep 5;154(6):745–753. doi: 10.1530/REP-17-0421

Figure 4. Effect of cell signaling inhibition on GDF9 and BMP15 induced AmhmRNA levels.

Figure 4

Cumulus cells were treated for 1 hour with the following specific SB431542 (SMAD2/3; 10 μM), or LDN-193189 (SMAD1/5/8; 100 nM) followed by a 48-hour co-treatment with GDF9 and BMP15 (5 ng/ml of each). Amh mRNA levels were determined 48 h after by qPCR and expressed relative to Rpl19. Columns with different letters differ significantly a–b P<0.001, one-way ANOVA, Bonferroni test, n=9.