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Mechanism behind MRI CEST. 1 Reporter gene, such as lysine-rich protein (LRP), is integrated within stem cell’s genome. 2 LRP is expressed and synthesized inside the cell. 3 Amide protons in LRP are irradiated, causing them to be switched with the surrounding ones in the water molecule. 4–5 Protons are transferred and the new water molecule generates reduced signaling on MRI, which corresponds to a change in contrast
