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. 2017 Sep 15;108(11):2295–2305. doi: 10.1111/cas.13379

Figure 4.

Figure 4

YM155 modulates human angiosarcoma cell proliferation through inhibition of nuclear survivin expression. (a) ISO‐HAS‐B human angiosarcoma cells cultured in endothelial cell media containing YM155 (100 [black square] and 500 nM [black inverted triangle]) exhibited evidently suppressed cell proliferation as compared with control (0 nM [gray triangle]). All data are means ± SD from triplicate experiments. (b) Representative F‐actin, survivin and merged immunofluorescence micrographs of ISO‐HAS‐B cells treated with YM155 (0 nM, top row; 100 nM, middle row; 500 nM, bottom row). ISO‐HAS‐B cells treated with YM155 (100 and 500 nM) showed pronounced decreased nuclear survivin expression and exhibited abnormal stellate‐like morphology. Scale bars, 100 μm. (c) ISO‐HAS‐B cells were cultured in endothelial cell media containing YM155 (0 nM [gray triangle] and 500 nM [black inverted triangle]) from 96 (arrow) to 240 h after plating, illustrating the efficacy of YM155 in suppression of cell proliferation at sub‐confluent cultures. All data are means ± SD from triplicate experiments.