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. 2017 Oct 1;7(10):1996–2008.

Figure 3.

Figure 3

Down-regulation of miR19b-3p inhibits colorectal cancer (CRC) cell metastasis. A. HCT116 and SW480 cell transplantation with miR19b-3p inhibitor or miR-NC inhibitor was performed to evaluate the cell migration ability. Data are expressed as mean ± SD. **P < 0.01 compared with the miR-NC inhibitor. B. The invasive abilities of HCT116 and SW480 cells were assessed by the Transwell invasion assay. Quantitative statistics for invasive cells are shown on the right panel. Data are expressed as mean ± SD. **P < 0.01 compared with miR-NC inhibitor. C. HCT116 and SW480 cells were transfected with the miR19b-3p inhibitor or miR-NC inhibitor, and western blotting (upper panel) and qRT-PCR (lower panel) assays were used to detect the expression of E-cadherin, N-cadherin, and vimentin. D. HCT116 and SW480 cells were incubated with E-cadherin and N-cadherin. Cells were immunized with anti-rabbit FITC-conjugated secondary antibody and then stained with DAPI. The core is shown in blue, and the target protein is shown in green. The scale bar represents 50 μm. E. Representative hematoxylin and eosin staining of lung tissue from mice injected with miR-NC inhibitor cells and miR19b-3p inhibitor cells.