Figure 7.

Effect of miR19b-3p on colorectal cancer (CRC) cell growth and metastasis is blocked by ITGB8 knockdown. A. The levels of ITGB8 in were detected by qRT-PCR (right panel) and western blotting analysis (left panel). B. Cell proliferation activity was measured by MTT assay as described. C. CRC growth was measured by the colony formation assay as described in vitro. The image (upper panel) and quantitative analysis of tumor cells colonies (lower panel) are shown. Data are expressed as mean ± SD. **P < 0.05 compared to miR-NC. ##P < 0.01 compared to miR19b-3p. D. Cell migration ability was measured by the wound healing assay. The image (left panel) and quantitative analysis of the percentage of closure (right panel) are shown. E. Cell invasion was analyzed by the Transwell Matrigel invasion assay. The image (upper panel) and quantitative analysis of the total invasive cells (lower panel) are shown. Data are expressed as mean ± SD. **P < 0.05 compared to miR-NC. ##P < 0.01 compared to miR19b-3p.