Figure 2.

NBAT1 suppresses OS cell proliferation, migration and invasion in vitro. A. MG63 cells were transfected with lentiviral particles expressing empty vector (con) and NBAT1, and the relative expression of NBAT1 was detected by RT-PCR. B. U2OS cells were transfected with lentiviral particles expressing scamble shRNA (shcon) and NBAT1 shRNA, and the relative expression of NBAT1 was detected by RT-PCR. C. The cell proliferation of control and NBAT1 overexpressed MG63 cells was determined by CCK-8 assay. D. The cell proliferation of control and NBAT1 silenced U2OS cells was determined by CCK-8 assay. E. The representitave images (left) and number (right) of the migrated and invaded MG63 cells in the control and NBAT1-overexpressed group. F. The representitave images (left) and number (right) of the migrated and invaded U2OS cells in the control and NBAT1-silenced group. All experiments were repeated three times. *p<0.05.