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. 2017 Oct 1;7(10):2009–2019.

Figure 4.

Figure 4

NBAT1 is physically associated with miR-21. A. Schematic outlining the predicted binding sites of miR-21 on NBAT1 or NBAT1-mut. B. Luciferase activity in U2OS cells cotransfected with miR-NC or miR-21 and luciferase reporters containing nothing, NBAT1 or NBAT1 mutant (NBAT1-mut) transcript. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. C. The RIP assay was performed to pull down the miRNAs associated with NBAT1. D. MG63 cell lysates were incubated with biotin-labeled NBAT1; after pull-down, miR-21 was assessed by RT-PCR. E. Anti-AGO2 RIP was performed in U2OS cells transiently overexpressing miR-NC or miR-21, followed by RT-PCR to detect NBAT1 associated with AGO2. F. The relative expression of NBAT1 in U2OS cells transfected with miR-NC or miR-21 was detected by RT-PCR. G. The relative expression of miR-21 in control or NBAT1 or NBAT1-mut overexpressed MG63 cells. H. The cellular proliferation of MG63 cells expressing NBAT1 with and without miR-21. I. The migration and invasion of MG63 cells expressing NBAT1 with and without miR-21. All experiments were repeated three times. *p<0.05.