Figure 4.
Effects of enzalutamide on urothelial tumor formation in mouse xenograft models. SVHUC-control or SVHUC-AR exposed to MCA and subsequently cultured for 6 weeks in the presence of ethanol (mock), hydroxyflutamide (HF; 5 μM), bicalutamide (BC; 5 μM), or enzalutamide (EZ; 5 μM) was suspended, mixed with Matrigel (1 × 106 cells/100 μL), and subcutaneously implanted into the flank of 6-week-old male NOD-SCID mice (n = 8 in each group). The endpoint for this study was tumor formation (exceeding 10 mm3 in its estimated volume [by the following formula: (short diameter)2 × (longest diameter) × 0.5] or 5 mm in greatest dimension). Comparisons were made by log-rank test.