Table 1.
Gene | Sense | Anti-sense | Product size (bp) |
---|---|---|---|
p53 | 5’-GGAGGGGCGATAAATACC-3’ | 5’-AACTGTAACTCCTCAGGCAGGC-3’ | 132 |
p27 | 5’-CGAGTGGCAAGAGGTGGAGA-3 | 5’-GGAGCCCCAATTAAAGGCG-3’ | 144 |
PTEN | 5’-GTTTACCGGCAGCATCAAAT-3’ | 5’-CCCCCACTTTAGTGCACAGT-3’ | 197 |
c-myc | 5’-ACCAGATCCCGGAGTTGGAA-3’ | 5’-CGTCGTTTCCGCAACAAGTC-3’ | 136 |
Cyclin D1 | 5’-GTGCTGCGAAGTGGAAACC-3’ | 5’-ATCCAGGTGGCGACGATCT-3’ | 174 |
Cyclin E | 5’-GCCAGCCTTGGGACAATAATG-3’ | 5’-CTTGCACGTTGAGTTTGGGT-3’ | 104 |
GAPDH | 5’-AAGGTGAAGGTCGGAGTCAAC-3’ | 5’-GGGGTCATTGATGGCAACAATA-3’ | 102 |