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. 2017 Oct 1;7(10):2070–2080.

Figure 6.

Figure 6

MiR-488 inhibits HULC expression by sponging to HULC in HCC cells. A. The relative HULC expression after transfection with miR-NC, miR-488 mimic, and miR-488 inhibitor into MHCC97H cells was analyzed by qRT-PCR. B. The relative HULC and miR-488 expression after transfection with si-NC, si-HULC-1, and si-HULC-2 into MHCC97H cells was analyzed by qRT-PCR. C. The predicted binding bites between miR-488 and HULC mRNA through complementary base-pairs. The wild-type HULC (HULC-WT) or mutant-type HULC (HULC-MUT) vectors containing the binding sites of miR-488 were constructed. D. The dual-luciferase reporter assay showed that miR-488 mimic significantly inhibited the luciferase activity of the HULC-WT binding site, whereas luciferase activity was not significantly changed in the HULC-MUT binding site. E, F. The cell colony number was evaluated after transfection with miR-NC, miR-488 inhibitor, miR-488 inhibitor, and si-HULC into MHCC97H cells. G, H. The cell invasion assay and invasion number were evaluated after transfection with miR-NC, miR-488 inhibitor, miR-488 inhibitor, and si-HULC into MHCC97H cells. The results are the average value of three independent tests. *P < 0.05; n.s., not significant.