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. 2017 Nov 1;7:14833. doi: 10.1038/s41598-017-12512-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SCARA5 is expressed in the stromal vascular fraction of white adipose tissue. (a) The C3H10T1/2 mesenchymal stem cell line and A33 committed stem cell line were compared to identify genes involved in adipocyte commitment. The genes with highly altered expression following commitment (indicated by fold change between A33 and C3H10T1/2 cells) are shown. The total gene expression dataset is available in Supplementary dataset 1. (b) Tissue distribution of SCARA5 expression using quantitative real-time PCR analysis (n = 3). (c) Real-time PCR of SCARA5 mRNA expression in the stromal vascular fraction (SVF) or fat fraction (FAT) prepared from the epididymal white adipose tissue of mice fed normal chow or a high-fat diet (HFD) (n = 3). (d) A33 and C3H10T1/2 cells were grown until post-confluence, total RNA was extracted, and real-time PCR was conducted to detect SCARA5 expression (n = 3). (e) A33 cells were differentiated, total RNA was isolated, and real-time PCR was conducted to detect SCARA5 expression at the indicated time points (n = 3). Quantitative data are presented as the mean ± SD. *P < 0.05; **P < 0.01 compared with the control.