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. 2017 Nov 1;7:14833. doi: 10.1038/s41598-017-12512-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of SCARA5 inhibits the commitment to adipocyte lineage and adipocyte differentiation. (a) The effects of SCARA5 silencing on commitment to the adipocyte lineage and subsequent adipocyte differentiation were assessed on day 5 by Oil red-O staining. The efficiency of the siRNA knockdown was assessed by RT-PCR on day 2 after transfection. (b) Proteins collected at the indicated times after induction were used to assay SCARA5, C/EBPα, and PPARγ2 levels with β-actin as the loading control. (c) SCARA5, C/EBPα, PPARγ2, and aP2/422 mRNA levels were measured in the scramble (sc) or si-SCARA5 cells using real-time PCR. (d) C3H10T1/2 cells were infected with MSCV or MSCV-SCARA5. After reaching post-confluence, cells were induced to differentiate using an adipocyte differentiation protocol and Oil red-O staining and triglyceride content was measured using spectrometric analysis on day 5 following induction. (e) Cells were harvested at the indicated times, and western blotting analysis was performed for SCARA5, FLAG, C/EBPβ, C/EBPα, and PPARγ using β-actin as the loading control. Quantitative data are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01 compared with the control.