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. 2017 Nov 1;7:14833. doi: 10.1038/s41598-017-12512-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The function of SCARA5 in adipocyte lineage commitment involves FAK-ERK. A33 cells were transfected with si-scramble and si-SCARA5 and differentiated using IBMX, insulin, and dexamethasone (MDI). (a) Cell counts were determined at 0 and 48 h after induction. **P < 0.01. Quantitative data are presented as the mean ± SD (n = 3). (b) DNA contents were analyzed using flow cytometry at the indicated time points. The suppression of SCARA expression abolished mitotic clonal expansion with a notable decrease in the entry of the cells to the G1-S phase. (c) SCARA5, pFAK (Tyr397), FAK, pERK, ERK, pAKT, and AKT were detected by western blotting at the indicated time points. The relative intensity of the western blots was determined in three independent experiments. (d) C3H10T1/2 cells were infected with MSCV or MSCV-SCARA5. After reaching post-confluence, cells were induced to differentiate using an adipocyte differentiation protocol. The expression of SCARA5, FLAG, pFAK (Tyr397), FAK, pERK, ERK, pAKT, and AKT were detected using western blotting at the indicated time points with β-actin as a loading control. The relative intensity of the western blots was determined in three independent experiments.