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. 2017 Nov 1;7:14833. doi: 10.1038/s41598-017-12512-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dexamethasone induces SCARA5 during adipocyte lineage commitment. (a) A33 cells were treated for 24 h with individual components of the differentiation cocktail (-, without MDI; M, IBMX; D, dexamethasone; I, insulin). Western blotting (upper) and real-time PCR (lower) were performed to confirm the expression of SCARA5. Quantitative data are presented as the mean ± SD (n = 3). **P < 0.01 compared with the control. (b) SCARA5 was induced using the modified protocol in proliferating C3H10T1/2 cells treated with dexamethasone. Dexamethasone treatment was performed during the proliferative stage, and MDI and rosiglitazone were administered 2-days post-confluence, designated as Day 0. (c) The cells were stained with Oil red-O after 7 days of differentiation. (d) SCARA5 protein and mRNA were detected by western blotting (upper) and real-time PCR analysis (lower left). The relative intensity of the western blots was determined in two independent experiments (lower right). Quantitative data are presented as the mean ± SD (n = 3). **P < 0.01 compared with the control. (e) Cells pretreated with dexamethasone were differentiated using differentiation medium. The cells were harvested at the indicated times, and western blotting of C/EBPβ, C/EBPα, and PPARγ was performed (^*NS, non-specific bands).