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. 2017 Nov 1;7:14833. doi: 10.1038/s41598-017-12512-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dexamethasone activation of the SCARA5 promoter is mediated by a glucocorticoid response element (GRE) motif. (a) SCARA5 promoter-luciferase constructs under basal conditions (10% CS). C3H10T1/2 cells were co-transfected with SCARA5 promoter-luciferase constructs and a Renilla-luciferase control vector. Promoter activity was normalized to Renilla luciferase activity. (b) The effect of 2 μM dexamethasone (Dex) on the activity of the SCARA5-luciferase constructs in C3H10T1/2 cells. Luciferase activity was measured 24 h after treatment with vehicle or dexamethasone. (c) The sequence of the SCARA5 promoter region containing the GRE (−641 to + 49). The wild-type (wt) putative GRE motif is marked in bold and the mutant (mut) GRE (−641 to −632) sequences are underlined. (d) The results of co-treatment with 1 μM RU486 on 2 μM dexamethasone-induced SCARA5 promoter-luciferase constructs containing wild-type and mutant versions of the GRE. (e) ChIP assay was performed using anti-GR antibodies or rabbit IgG. The promoter region (−641 to −632) of SCARA5 with putative GRE was amplified by real-time PCR. The positive control is Dexras1 promoter⁴⁴, and the negative control is >1 kb upstream of SCARA5 promoter. Quantitative data are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01 compared with the control.