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. 2017 Oct 31;7:14701. doi: 10.1038/s41598-017-14822-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cardioprotective effect of MLC901 on isolated hearts. (A) C57Bl6 mouse hearts were mounted on a Langendorff system. After a 20 minutes period of stabilization, global ischemia was induced during 30 minutes. Reperfusion was achieved by restoring the flow during 60 minutes with the Krebs solution. MLC901 was administered during reperfusion at 3 different concentrations: 5, 50 and 500 ng/ml. (B) Scatter dot blots (means ± SD) were represented for infarct size (in % of LV) in IR (n = 7), MLC901 (5 ng/ml, n = 6), MLC901 (50 ng/ml, n = 6), MLC901 (500 ng/ml; n = 6). Representative pictures of TTC-stained LV slices were shown for each group. Statistical analysis was performed using ANOVA with the Tukey’s post hoc test for multiple comparisons.