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. 2017 Oct 31;7:14701. doi: 10.1038/s41598-017-14822-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cardioprotection is mediated by an inhibition of apoptosis in vivo. (A) Specific DNA fragmentation was quantified after 1h-reperfusion. Injection of MLC901 (0.4, 4 and 40 µg/kg) was performed intravenously 5 minutes before the onset of reperfusion. Scatter dot blots and means ± SD were plotted for the ratio of internucleosomal DNA fragmentation measured in I versus NI regions of LV from IR or MLC901-treated mice. (B) Scatter dot blots and means ± SD were plotted for the ratio of internucleosomal DNA fragmentation measured in I versus NI regions of LV after 24h-reperfusion in mice not treated (IR_24h) or treated by MLC901 (4 µg/kg). (C–H) Western blot analysis of pJNK, JNK, pERK1/2, ERK1/2, pAkt and Akt was performed on LV from MLC901 treated versus non-treated IR mice. (C,E,G) Representative gel blots for LV protein extract were cropped from initial blots presented in Supplementary Figures S5, S6 and S7. Histograms (means ± SD) were plotted for (D) pJNK/JNK, (F) pERK1/2/ERK1/2 and (H) pAKT/AKT ratios for MLC901 (n = 7) versus IR (n = 7); Statistical analysis was performed using ANOVA with the Tukey’s post hoc test for multiple comparisons or using the Mann-Whitney test for comparison between two groups.