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. 2017 Nov 1;8:1249. doi: 10.1038/s41467-017-01359-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — RNA-seq after miRNA cocktail exposures shows myogenic-specific comittment enhancement of transcriptional profile. a PC analysis of a MAB-MiPs samples reveals stage-specific, progeny-specific clustering. b Unbiased clustering of MAB-MiPs analyzed by RNA-seq. c–f Volcano plots of fold change vs p-value of all DE genes in different conditions (threshold, P = 0.05, dashed line). MAB-MiPs untreated vs MAB-MiPs+AMC c, MAB-MiPs untreated vs MAB-MiPs+PMC d, fibroblast-MiPs untreated vs fibroblasts-MiPs+AMC e, fibroblast-MiPs untreated vs fibroblasts-MiPs+PMC f. Left side of all charts, all genes and highlighted candidates enriched in untreated-MiPs; right side, genes and candidates enriched in treated-MiPs. Blue dots depict genes that were found downregulated while red dots depict genes that were found upregulated upon treatments. g Quantitation of results obtained from bisulfite sequencing of upstream CpG islands of selected anti-myogenic and pro-myogenic gene pools in treated and untreated cells. Data is depicted as heatmap of average percentages of methylated CpGs. h Quantitation of results obtained from ChIP-qPCR experiments analyzing fibroblast- and MAB-MiPs treated and untreated. H3k9me3, repressive marker; H3k4me2, H3k27ac, permissive markers. Data is depicted as heatmap of average percentages of IP-enriched vs total input DNA (n = 3). f-, fibroblast-derived. AMC, anti-myogenic cocktail (miRNA-based), PMC, pro-myogenic cocktail (miRNA-based)