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. 2017 Nov 1;7:14811. doi: 10.1038/s41598-017-13694-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterization of endothelial cells derived from H9 human embryonic stem cells. (A) Typical morphology of undifferentiated H9 hESCs. Scale bar, 200 μm. (B) Pluripotent staining of H9 hESCs using OCT4 (Green), SOX2 (Red), NANOG (Green) and SSEA4 (Red). DAPI indicates nuclear staining (Blue). Scale bar, 100 μm. (C) Typical morphology of H9-ECs. Scale bar, 200 μm. (D) FACS analysis of CD144-positive cells. (E) CD144 (Green) staining of H9-ECs. DAPI indicates nuclear staining (Blue). Scale bar, 50 μm. (F) Dil-ac-LDL (Red) staining of H9-ECs. DAPI indicates nuclear staining (Blue). Scale bar, 100 μm.