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. 2017 Nov 1;7:14801. doi: 10.1038/s41598-017-14942-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nrdc transcriptionally regulates cell cycle-associated genes via the modulation of histone acetylation. (A, B) RT-PCR of the indicated cell cycle-associated genes in Nrdc+/+ and Nrdc−/− iMEF (A), and in Nrdc−/−^C, Nrdc−/−^WT, and Nrdc−/−^EA iMEF (B) (n = 3 per group). (C) ChIP-PCR using anti-Nrdc or control IgG shows Nrdc enrichment at the promoters of the indicated Nrdc direct targets in Nrdc+/+ iMEF (n = 3 per group). (D,E) ChIP-PCR analysis using anti-H3K4me2 and control IgG in Nrdc+/+ and Nrdc−/− iMEF (D), and in Nrdc−/−^C, Nrdc−/−^WT, and Nrdc−/−^EA iMEF (E) for the promoters of the indicated Nrdc target genes (n = 3 per group). (F,G) ChIP-PCR using anti-H3K9ac in Nrdc+/+ and Nrdc−/− iMEF (F), and in Nrdc−/−^C, Nrdc−/−^WT, and Nrdc−/−^EA iMEF (G) for the promoters of the indicated Nrdc target genes (n = 3 per group). All error bars indicate the standard error (S.E). *Indicates p < 0.05 between either Nrdc+/+ v.s. Nrdc−/− or Nrdc−/−^C v.s. Nrdc−/−^WT. ^†Indicates P < 0.05 Nrdc−/−^C v.s. Nrdc−/−^EA.