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. 2017 Nov 1;7:14793. doi: 10.1038/s41598-017-14933-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of sea bass derived hepcidins on ferroportin from mouse macrophages. Mouse bone marrow-derived macrophages were treated with sea bass Hamp1 or Hamp2 peptides for 1, 6 and 24 hours, after which (A) fpn1 mRNA expression and (B) Fpn1 protein levels were measured by real-time PCR and Western blot, respectively. Values are expressed as the means ± SD of three independent experiments. The Western blot images were obtained from 3 different blots (for 1, 6 and 24 hours) and each is representative of 3 independent experiments. Uncropped blots are presented in Supplementary Figure 5.