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. 2017 Nov 1;7:14836. doi: 10.1038/s41598-017-13865-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic fingerprint of exemplary intracellular nanostructures. (A) Representative confocal images of clathrin-coated vesicles, macropinosomes, and caveolae, respectively (green signals). The blue signal indicates the nucleus. Scale bar: 10 µm. (B) Representative iMSD plots of the three structures with error bars (clathrin-coated vesicles in blue, macropinosomes in green, and caveolae in red). (C) 3D plot of extracted parameters shows the differences in the dynamic (short-range diffusivity and anomalous coefficient) and structural (size) properties of the selected structures. (D) Representative confocal images of early endosomes, late endosomes, and lysosomes, respectively (green signals). Scale bar: 10 µm. (E) Representative iMSD plots of the three structures with error bars (early endosomes in dark yellow, late endosomes in orange, and lysosomes in grey). (F) 3D plot of extracted parameters shows the differences in the dynamic and structural properties of the selected structures.