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. 2017 Nov 1;7:14836. doi: 10.1038/s41598-017-13865-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic fingerprint of the lysosome at different spatiotemporal scales. (A) Exemplary iMSD plots of the lysosome at three different temporal scales: short, 0–0.5 seconds (left, light grey); intermediate, 0–12 seconds (middle, grey); long, 0–60 seconds (right, dark grey). (B) 3D plot of the dynamic fingerprint of the lysosome at the three different temporal scales. (C) The first 6 seconds of the iMSD plot are reported here (upper panel) to show the goodness of the fit to the global model (red line and residuals, Eq. 6 in Materials and Methods). The derivative of the iMSD plot shows the trend of the measured diffusivity (lower panel).