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. 2017 Nov 1;7:14873. doi: 10.1038/s41598-017-14115-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Left panel: square root of the amplitude variance from within a ROI window of 0.08 mm in depth, ~3mm in width, moved in depth (red circles) and mean amplitude of the OCT signal (black line) of sample A. A single scaling factor (𝛼) is used to scale the square root of the amplitude variance to the mean amplitude. Panels on the right side shows the histograms of the amplitude distributions within the ROI from points a-c as indicated with arrows in the left panel. The amplitude distributions are fitted with a Rayleigh distribution (equation (3)), giving the reported contrast, speckle variance and R² of the fits. The amplitude stays Rayleigh distributed when the ROI is moved in depth, until regions of very low SNR are reached (point c).